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Signal Transduction and Biotechnology Lab

Protein Chemistry and Structural Biology laboratory is dedicated for protein research. The lab is equipped with AKTA and FPLC AKTA fraction collectors, Nanodrop spectrophotometer, Plate reader spectrofluorimeter and Circular Dichroism Spectropolarimeter. The lab is intensively used to purify and characterize different protein/peptide samples from SU laboratories or from other research groups. The lab is dedicated for recombinant DNA cloning, heterologous protein expression, protein purification and biophysical characterization. The lab is equipped with laminar flow cabinet, thermocycler for PCR, benchtop centrifuges, pH meter and other small equipment used in basic protein research. Furthermore there are shakers and incubators, DNA and protein electrophoresis equipment, high speed and ultra high speed centrifuges, spectrophotometers, DNA sequencer and scintillation counter for common use. Current research projects focus on metal binding and heterotrimeric GTP-binding proteins of plant origin. Different biochemical and biophysical characterization techniques are investigated including Small Angle X-ray Scattering, CD, UV spectrophotometric analysis, radioactive and fluorometeric enzyme assays along with other standart assays.