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With the developments in molecular biology and
DNA sequencing technologies, some biological
problems can be addressed genome-wide. After Next-
generation Sequencing (NGS) technology was
established, the cost of DNA sequencing has been
decreasing which leads molecular experiments to
change their form. These experiments can now be
designed to solve DNA-related problems at the
genome scale. Consequently, a number of new NGS-
based technologies are developed every year.
Massively accumulating sequence datasets are often
reanalyzed with the aim of answering related but
distinct questions from different perspectives.
Therefore, it is important to understand not only how
these techniques work and but also how their
outcomes are in silico analyzed. This course will
cover a part of available sequencing techniques such
as whole genome sequencing, exome sequencing,
RNA-seq, GRO-seq, NET-seq, ChIP-seq, DNase-seq,
FAIRE-seq, Hi-C-seq, XR-seq, Damage-seq, etc. The
students will learn how to perform these techniques
theoretically and how to analyze them practically. The
students will be assigned to read and present related
articles. Students will also perform an NGS-related
project with the skills they will learn during the course
flow.
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