Tissue Engineering (BIO 421)

2023 Fall
Faculty of Engineering and Natural Sciences
Sibel Çetinel cetinel@sabanciuniv.edu,
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BIO301 BIO332
Interactive lecture,Laboratory
Interactive,Discussion based learning,Project based learning
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Tissue engineering combines the skills of engineering and knowledge of principle biology to generate, restore and replace damaged tissues and organs. To engineer living tissues mimicking conditions in living organism is essential. Therefore, tissue engineering is considered a biomedical engineering discipline and a potential alternative to tissue and organ transplantation. This course is built on three main pillars of tissue engineering: cells, scaffolds, and growth factors. Initially stem cells and differentiation is discussed as well as cell-based tissue engineering applications. Then design and characterization of biomaterials and nanomaterials as tissue scaffolds are covered. Here, various bio-fabrication techniques including 3D bioprinting are detailed. This course also covers the interaction with biomaterial surface, mechanical loading, biologic regulators, and culture conditions. Finally, examples of tissue engineering- based procedures that can alleviate specific diseases and clinical translation of regenerative therapies are analyzed as case studies with student presentations. This course also contains a laboratory session. At this session students will learn to handle mammalian cell cultures, prepare hydrogel and polymer scaffolds, perform tissue culture and characterization.


Students will learn certain aspects of tissue engineering and how to utilize this information to generate overall strategic approach used to solve a clinical problem.


1. Understand the world, their country, their society, as well as themselves and have awareness of ethical problems, social rights, values and responsibility to the self and to others.

2. Understand different disciplines from natural and social sciences to mathematics and art, and develop interdisciplinary approaches in thinking and practice.

3. Think critically, follow innovations and developments in science and technology, demonstrate personal and organizational entrepreneurship and engage in life-long learning in various subjects; have the ability to continue to educate him/herself.

4. Communicate effectively in Turkish and English by oral, written, graphical and technological means.

5. Take individual and team responsibility, function effectively and respectively as an individual and a member or a leader of a team; and have the skills to work effectively in multi-disciplinary teams.

1. Possess sufficient knowledge of mathematics, science and program-specific engineering topics; use theoretical and applied knowledge of these areas in complex engineering problems.

2. Identify, define, formulate and solve complex engineering problems; choose and apply suitable analysis and modeling methods for this purpose.

3. Develop, choose and use modern techniques and tools that are needed for analysis and solution of complex problems faced in engineering applications; possess knowledge of standards used in engineering applications; use information technologies effectively.

4. Have the ability to design a complex system, process, instrument or a product under realistic constraints and conditions, with the goal of fulfilling specified needs; apply modern design techniques for this purpose.

5. Design and conduct experiments, collect data, analyze and interpret the results to investigate complex engineering problems or program-specific research areas.

6. Possess knowledge of business practices such as project management, risk management and change management; awareness on innovation; knowledge of sustainable development.

7. Possess knowledge of impact of engineering solutions in a global, economic, environmental, health and societal context; knowledge of contemporary issues; awareness on legal outcomes of engineering solutions; knowledge of behavior according to ethical principles, understanding of professional and ethical responsibility.

8. Have the ability to write effective reports and comprehend written reports, prepare design and production reports, make effective presentations, and give and receive clear and intelligible instructions.

1. Comprehend key concepts in biology and physiology, with emphasis on molecular genetics, biochemistry and molecular and cell biology as well as advanced mathematics and statistics.

2. Develop conceptual background for interfacing of biology with engineering for a professional awareness of contemporary biological research questions and the experimental and theoretical methods used to address them.

1. Applying fundamental and advanced knowledge of natural sciences as well as engineering principles to develop and design new materials and establish the relation between internal structure and physical properties using experimental, computational and theoretical tools.

2. Merging the existing knowledge on physical properties, design limits and fabrication methods in materials selection for a particular application or to resolve material performance related problems.

3. Predicting and understanding the behavior of a material under use in a specific environment knowing the internal structure or vice versa.

1. Familiarity with concepts in statistics and optimization, knowledge in basic differential and integral calculus, linear algebra, differential equations, complex variables, multi-variable calculus, as well as physics and computer science, and ability to use this knowledge in modeling, design and analysis of complex dynamical systems containing hardware and software components.

2. Ability to work in design, implementation and integration of engineering applications, such as electronic, mechanical, electromechanical, control and computer systems that contain software and hardware components, including sensors, actuators and controllers.


  Percentage (%)
Midterm 30
Group Project 20
Presentation 20
Other 30



Jan de Boer, Clemens van Blitterswijk (2022, Third Edition), Tissue Engineering, Academic Press. ISBN: 978-0-12-824459-3
Robert Lanza, Robert Langer, Joseph P. Vacanti, Anthony Atala (2020, Fifth Edition), Principles of Tissue Engineering, Academic Press. ISBN: 978-0-12-818422-6