Plant Tissue Culture Techniques (BIO 445)

2022 Fall
Faculty of Engineering and Natural Sciences
Mol.Bio.Genetic&Bioengin.(BIO)
3
6/7 ECTS (for students admitted in the 2013-14 Academic Year or following years)
Selim Çetiner cetiner@sabanciuniv.edu,
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English
Undergraduate
--
Formal lecture,Interactive lecture,Laboratory
Interactive,Case Study
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CONTENT

This is a practical course on both principles and practices of plant tissue culture techniques. The laboratory organization and requirements; principles of plant cell, tissue, and organ cultures; organic and inorganic components of the plant tissue culture media; physiology of in vitro grown plants and acclimatization; meristem culture; cell suspension culture; somatic emryogenesis; organogenesis; adventitious shoot and root formation; commercial applications will be covered in detail.

OBJECTIVE

Refer to the course content

LEARNING OUTCOMES

  • Botanical basis of plant tissue culture techniques
  • Importance of asceptic plant material and laboratory conditions for tissue culture
  • The components of various plant tissue culture media and hormones
  • Applications of various in-vitro tissue culture techniques for commercial plant propagation.
  • Economic considerations for plant tissue culture operations

PROGRAMME OUTCOMES


1. Understand the world, their country, their society, as well as themselves and have awareness of ethical problems, social rights, values and responsibility to the self and to others. 3

2. Understand different disciplines from natural and social sciences to mathematics and art, and develop interdisciplinary approaches in thinking and practice. 5

3. Think critically, follow innovations and developments in science and technology, demonstrate personal and organizational entrepreneurship and engage in life-long learning in various subjects; have the ability to continue to educate him/herself. 5

4. Communicate effectively in Turkish and English by oral, written, graphical and technological means. 5

5. Take individual and team responsibility, function effectively and respectively as an individual and a member or a leader of a team; and have the skills to work effectively in multi-disciplinary teams. 5


1. Possess sufficient knowledge of mathematics, science and program-specific engineering topics; use theoretical and applied knowledge of these areas in complex engineering problems. 4

2. Identify, define, formulate and solve complex engineering problems; choose and apply suitable analysis and modeling methods for this purpose. 4

3. Develop, choose and use modern techniques and tools that are needed for analysis and solution of complex problems faced in engineering applications; possess knowledge of standards used in engineering applications; use information technologies effectively. 4

4. Have the ability to design a complex system, process, instrument or a product under realistic constraints and conditions, with the goal of fulfilling specified needs; apply modern design techniques for this purpose. 4

5. Design and conduct experiments, collect data, analyze and interpret the results to investigate complex engineering problems or program-specific research areas. 4

6. Possess knowledge of business practices such as project management, risk management and change management; awareness on innovation; knowledge of sustainable development. 5

7. Possess knowledge of impact of engineering solutions in a global, economic, environmental, health and societal context; knowledge of contemporary issues; awareness on legal outcomes of engineering solutions; knowledge of behavior according to ethical principles, understanding of professional and ethical responsibility. 5

8. Have the ability to write effective reports and comprehend written reports, prepare design and production reports, make effective presentations, and give and receive clear and intelligible instructions. 4


1. Comprehend key concepts in biology and physiology, with emphasis on molecular genetics, biochemistry and molecular and cell biology as well as advanced mathematics and statistics. 5

2. Develop conceptual background for interfacing of biology with engineering for a professional awareness of contemporary biological research questions and the experimental and theoretical methods used to address them. 4


1. Use mathematics (including derivative and integral calculations, probability and statistics, differential equations, linear algebra, complex variables and discrete mathematics), basic sciences, computer and programming, and electronics engineering knowledge to (a) Design and analyze complex electronic circuits, instruments, software and electronics systems with hardware/software or (b) Design and analyze communication networks and systems, signal processing algorithms or software 1


1. Applying fundamental and advanced knowledge of natural sciences as well as engineering principles to develop and design new materials and establish the relation between internal structure and physical properties using experimental, computational and theoretical tools. 2

2. Merging the existing knowledge on physical properties, design limits and fabrication methods in materials selection for a particular application or to resolve material performance related problems. 2

3. Predicting and understanding the behavior of a material under use in a specific environment knowing the internal structure or vice versa. 2

ASSESSMENT METHODS and CRITERIA

  Percentage (%)
Final 50
Midterm 25
Individual Project 25

RECOMENDED or REQUIRED READINGS

Textbook

Plants from Test Tubes by L. Kyte and J. Kleyn, 1996.
Micropropagation by P. Debergh and R. H. Zimmerman, 1994