Plant Tissue Culture Techniques (BIO 445)

2022 Fall
Faculty of Engineering and Natural Sciences
Mol.Bio.Genetic&Bioengin.(BIO)
3
6.00 / 7.00 ECTS (for students admitted in the 2013-14 Academic Year or following years)
Selim Çetiner -cetiner@sabanciuniv.edu,
English
Undergraduate
--
Formal lecture,Interactive lecture,Laboratory
Interactive,Case Study
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CONTENT

This is a practical course on both principles and practices of plant tissue culture techniques. The laboratory organization and requirements; principles of plant cell, tissue, and organ cultures; organic and inorganic components of the plant tissue culture media; physiology of in vitro grown plants and acclimatization; meristem culture; cell suspension culture; somatic emryogenesis; organogenesis; adventitious shoot and root formation; commercial applications will be covered in detail.

OBJECTIVE

Refer to the course content

LEARNING OUTCOME

Botanical basis of plant tissue culture techniques
Importance of asceptic plant material and laboratory conditions for tissue culture
The components of various plant tissue culture media and hormones
Applications of various in-vitro tissue culture techniques for commercial plant propagation.
Economic considerations for plant tissue culture operations

ASSESSMENT METHODS and CRITERIA

  Percentage (%)
Final 50
Midterm 25
Individual Project 25

RECOMENDED or REQUIRED READINGS

Textbook

Plants from Test Tubes by L. Kyte and J. Kleyn, 1996.
Micropropagation by P. Debergh and R. H. Zimmerman, 1994