A general introduction to basic techniques used in characterization and separation of biological systems and molecules. Topics include microscopy, centrifugation, liquid chromatography methods, spectroscopy and electrophoresis. Lab sessions will give a chance to use these techniques individually.
Analytical Techniques (BIO 335)
Programs\Type | Required | Core Elective | Area Elective |
BA- Political Science | |||
BA-Cultural Studies | |||
BA-Cultural Studies | |||
BA-Economics | |||
BA-Economics | |||
BA-International Studies | |||
BA-International Studies | |||
BA-Management | |||
BA-Management | |||
BA-Political Sci.&Inter.Relat. | |||
BA-Political Sci.&Inter.Relat. | |||
BA-Social & Political Sciences | |||
BA-Visual Arts&Visual Com.Des. | |||
BA-Visual Arts&Visual Com.Des. | |||
BS-Biological Sci.&Bioeng. | * | ||
BS-Computer Science & Eng. | |||
BS-Computer Science & Eng. | |||
BS-Electronics Engineering | * | ||
BS-Electronics Engineering | * | ||
BS-Industrial Engineering | |||
BS-Manufacturing Systems Eng. | |||
BS-Materials Sci. & Nano Eng. | * | ||
BS-Materials Science & Eng. | * | ||
BS-Mechatronics | |||
BS-Mechatronics | |||
BS-Microelectronics | |||
BS-Molecular Bio.Gen.&Bioeng | * | ||
BS-Telecommunications | * |
CONTENT
OBJECTIVE
To teach students the basic concepts of bioanalytical techniques in both, theory and practice, which are relevant to biologists and bioengineers but also students from different fields.
LEARNING OUTCOME
Upon completion of the course, students will have a general knowledge of basic laboratory techniques for characterization and separation of biological systems and molecules. Students would be able to perform basic laboratory techniques.
Module 1 Microscopy: Students will have a deeper understanding of both, theory and application in the following topics:
Basic Concepts in Microscopy
Light microscopy
Fluorescence microscopy
Confocal Microscopy
Live-cell imaging and Sensor techniques
Module 2 Electrophoresis Techniques: Students will learn how to use Gel electrophoresis techniques for analysis of nucleic acids and proteins for both, diagnostic purposes and preparative purposes, which includes:
Basic Concepts in Electrophoresis
Horizontal and Vertical Electrophoresis
2D Gel Electrophoresis and Protein Detection Methods
Electrophoresis of Nucleic Acids
Module 3 Centrifugation Techniques:
Basic Concepts of Centrifugation Techniques
Types of Centrifuges and analytical ultracentrifugation techniques
Separation methods and preparative ultracentrifuges
Types of rotors
Module 4 Spectroscopic Techniques:
Introduction and basic concepts of Spectroscopic Techniques
UV-VIS Spectroscopy
Infrared and fluorescence spectroscopy
Circular dichroism (CD) spectroscopy
NMR Spectroscopy and X-ray crystallography
Atomic Spectroscopy and Mass spectroscopy
Module 5 PCR Techniques:
Principles of Polymerase Chain reaction
Preparative and Diagnostic PCR
DNA Sequencing methods
Sanger Sequencing, Next Generation Sequencing Methods
Module 6 Chromatography and Mass spectrometry:
Basic Principles of Chromatography
Thin layer chromatography
Liquid Chromatography
Gas Chromatography
Principles of MALDI-TOF
Electrospray Ionization Mass Spectrometry
Update Date:
ASSESSMENT METHODS and CRITERIA
Percentage (%) | |
Final | 30 |
Midterm | 30 |
Other | 40 |
RECOMENDED or REQUIRED READINGS
Textbook |
Bioanalytics: Analytical Methods and Concepts in Biochemistry and Molecular Biology |
Readings |
Course slides updated annually. |