Upon completion of the course, students will have a general knowledge of basic laboratory techniques for characterization and separation of biological systems and molecules. Students would be able to perform basic laboratory techniques.

Module 1 Microscopy: Students will have a deeper understanding of both, theory and application in the following topics:

Basic Concepts in Microscopy
Light microscopy
Fluorescence microscopy
Confocal Microscopy
Live-cell imaging and Sensor techniques

Module 2 Electrophoresis Techniques: Students will learn how to use Gel electrophoresis techniques for analysis of nucleic acids and proteins for both, diagnostic purposes and preparative purposes, which includes:

Basic Concepts in Electrophoresis
Horizontal and Vertical Electrophoresis
2D Gel Electrophoresis and Protein Detection Methods
Electrophoresis of Nucleic Acids

Module 3 Centrifugation Techniques:

Basic Concepts of Centrifugation Techniques
Types of Centrifuges and analytical ultracentrifugation techniques
Separation methods and preparative ultracentrifuges
Types of rotors

Module 4 Spectroscopic Techniques:

Introduction and basic concepts of Spectroscopic Techniques
UV-VIS Spectroscopy
Infrared and fluorescence spectroscopy
Circular dichroism (CD) spectroscopy
NMR Spectroscopy and X-ray crystallography
Atomic Spectroscopy and Mass spectroscopy

Module 5 PCR Techniques:

Principles of Polymerase Chain reaction
Preparative and Diagnostic PCR
DNA Sequencing methods
Sanger Sequencing, Next Generation Sequencing Methods

Module 6 Chromatography and Mass spectrometry:

Basic Principles of Chromatography
Thin layer chromatography
Liquid Chromatography
Gas Chromatography
Principles of MALDI-TOF
Electrospray Ionization Mass Spectrometry