Introduction to Molecular Biology (BIO 301)

2021 Spring
Faculty of Engineering and Natural Sciences
Mol.Bio.Genetic&Bioengin.(BIO)
4
8.00 / 7.00 ECTS (for students admitted in the 2013-14 Academic Year or following years)
Nur Mustafao─člu Varol nur.mustafaoglu@sabanciuniv.edu,
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English
Undergraduate
BIO321
Formal lecture,Interactive lecture,Group tutorial,Laboratory
Interactive,Learner centered,Communicative,Discussion based learning,Task based learning
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CONTENT

Introduction to Molecular Biology is a detailed introduction to basic mechanisms of molecular biology and genetics. Starting from genomic information and genome organization, DNA structure, packaging to chromosomes, replication of genetic material and its fidelity, mechanisms to repair and recombination of DNA will be discussed in the lecture. RNA structure, mechanisms of DNA transcription to RNA, transcriptional control mechanisms and transcription factors will be studied in detail. RNA to protein translation mechanisms and ribosome structure will be dissected. Protein stability, protein degradation echanisms, role of miRNAs and RNA stability on gene expression will be discussed. A general introduction to programmed cell death mechanims will be presented.The lab course is practical course on principle techniques for production of recombinant proteins in bacteria and animal cells. Lab work will involve utilisation of molecular cloning techniques to produce a particular gene product. Recombinant plasmids carrying either single gene or that for a fusion protein will be constructed and used to transform E. coli, and the gene products will be isolated and characterized.

OBJECTIVE

Course:
Introduction to the basic mechanisms of molecular biology and cellular functions.

Lab:
Introduction to the basic recombinant DNA technology theory, teaching cloning strategies and gene expression methods.

LEARNING OUTCOME

To successfully complete Intro. to Molecular Biology (BIO 301) students are expected to;
Gain a clear knowledge about cell cycle and its regulation.


Have an insight of gene and chromosme structure.
Have a general idea about chromosome remodelling.
Define the stages of DNA replication in eukaryotes and prokaryotes.
Define the types of mutations, DNA damage and repair mechanisms.
Clearly define the stages of transcription and translation.
Have a general idea about recombinant protein production via molecular cloning.

ASSESSMENT METHODS and CRITERIA

  Percentage (%)
Final 30
Midterm 30
Exam 10
Written Report 15
Homework 10
Other 5

RECOMENDED or REQUIRED READINGS

Textbook

Molecular Biology of the Gene, Seventh Edition, Pearson
J. D. Watson, T. A. Baker, S. P. Bell, A. Gann, M. Levine, R. Losick
ISBN-13: 9780321762436

Readings

- Molecular Biology Principles of Genome Function
N. Craig, R. Green, C. Greider, G. Storz, C. Wolberger, O. Cohen-Fix
ISBN-13: 978-0199658572
- Molecular Biology of the Cell
B. Alberts, A. D. Johnson, J. Lewis, D. Morgan, M. Raff, K. Roberts, P. Walter
ISBN-13: 978-0815344322

Optional Readings

Research articles
Review articles