Mammalian Cell Culture (BIO 370)

2020 Fall
Faculty of Engineering and Natural Sciences
6.00 / 7.00 ECTS (for students admitted in the 2013-14 Academic Year or following years)
Hüveyda Başağa,
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The aim of this course is to introduce the students to basic principles and techniques of mammalian cell culture and to provide essential information on the physiological and molecular aspects of the culture and growth of different cells. The course will be mainly demonstrations and laboratory exercises supplemented with lectures. Lecture topic areas cover molecular composition of cells, effects of media and physical parameters on growth, and metabolism. It also deals with regulation of gene expression as well as stress responses of cells, maintenance of energy and cell survival. The laboratory sessions will be conducted much as the students were working in a research laboratory therefore students will gain hands on experience in cellular and molecular biology techniques.


Refer to the course content


? Classroom sessions of this module aims to teach fundamental concepts in mammalian cell culture inluding topics on Biology of Cultured Cells, Lab Design and Equipment, Aseptic Technique and Safety, Media and Supplements, Primary Cultures and Cell Lines, Cloning and Selection, Transformation and Immortalization, and Cell Daeath to the students who do have at least D ?grade in freshman level Biology, Chemistry (NS 102 ? Science of Nature II) and Calculus (MATH 101 & MATH 102) courses.
? As a co-requisite the classrooms sessions, laboratory sessions of this module (BIO370L) aims to introduce major experimental tissue culture methods used in modern biology weekly by simultaneously complementing the in-class covered concepts.

After completing the in-class teaching and laboratory activities associated with this module you should be able to:
Module-specific skills
a) Identify the fundamental concepts of mammalian cell culture.
b) Describe the laboratory design and equipment suitable for cell culture.
c) Describe and apply the aseptic technique necessary for cell culture work.
d) Describe the cell culture basal media and supplements in general.
e) Describe and basically handle mammalian primary cultures and cell lines
f) Describe the main aspects of cloning, selection, transformation and immortalization in mammalian cell culture.

Discipline-specific skills
g) Develop a general understanding to approach essential facts and theories of mammalian cell culture.
h) Identify the key features of biology of cultured cells, aseptic technique, and subculture of primary cultures/cell lines and use these features in frame of a tissue culture experimental design and method in a laboratory context, with some guidance.
i) With some guidance, analyze, interpret and report experimentally derived data, which have lowered level of complexity than the ones gained from real world experimentations, in details.

Individual and key skills
j) Express basic tissue culture concepts effectively by written means in project and lab reports.
k) With some guidance, identify the covered concepts in the provided written sources and study autonomously.
l) With some guidance, collect specific information about important supplements to the cell cultures from various written sources and understand their roles in a cell culture laboratory method.


1. Understand the world, their country, their society, as well as themselves and have awareness of ethical problems, social rights, values and responsibility to the self and to others. 5

2. Understand different disciplines from natural and social sciences to mathematics and art, and develop interdisciplinary approaches in thinking and practice. 5

3. Think critically, follow innovations and developments in science and technology, demonstrate personal and organizational entrepreneurship and engage in life-long learning in various subjects. 5

4. Communicate effectively in Turkish and English by oral, written, graphical and technological means. 5

5. Take individual and team responsibility, function effectively and respectively as an individual and a member or a leader of a team; and have the skills to work effectively in multi-disciplinary teams. 5

1. Possess sufficient knowledge of mathematics, science and program-specific engineering topics; use theoretical and applied knowledge of these areas in complex engineering problems.

2. Identify, define, formulate and solve complex engineering problems; choose and apply suitable analysis and modeling methods for this purpose.

3. Develop, choose and use modern techniques and tools that are needed for analysis and solution of complex problems faced in engineering applications; possess knowledge of standards used in engineering applications; use information technologies effectively.

4. Ability to design a complex system, process, instrument or a product under realistic constraints and conditions, with the goal of fulfilling specified needs; apply modern design techniques for this purpose.

5. Design and conduct experiments, collect data, analyze and interpret the results to investigate complex engineering problems or program-specific research areas.

6. Knowledge of business practices such as project management, risk management and change management; awareness on innovation; knowledge of sustainable development.

7. Knowledge of impact of engineering solutions in a global, economic, environmental, health and societal context; knowledge of contemporary issues; awareness on legal outcomes of engineering solutions; understanding of professional and ethical responsibility.

1. Comprehend key concepts in biology and physiology, with emphasis on molecular genetics, biochemistry and molecular and cell biology as well as advanced mathematics and statistics.

2. Develop conceptual background for interfacing of biology with engineering for a professional awareness of contemporary biological research questions and the experimental and theoretical methods used to address them.

1. Applying fundamental and advanced knowledge of natural sciences as well as engineering principles to develop and design new materials and establish the relation between internal structure and physical properties using experimental, computational and theoretical tools.

2. Merging the existing knowledge on physical properties, design limits and fabrication methods in materials selection for a particular application or to resolve material performance related problems.

3. Predicting and understanding the behavior of a material under use in a specific environment knowing the internal structure or vice versa.

1. Use mathematics (including derivative and integral calculations, probability and statistics, differential equations, linear algebra, complex variables and discrete mathematics), basic sciences, computer and programming, and electronics engineering knowledge to (a) Design and analyze complex electronic circuits, instruments, software and electronics systems with hardware/software. or (b) Design and analyze communication networks and systems, signal processing algorithms or software