Analytical Techniques (BIO 335)

2019 Spring
Faculty of Engineering and Natural Sciences
Mol.Bio.Genetic&Bioengin.(BIO)
3
6.00 / 6.00 ECTS (for students admitted in the 2013-14 Academic Year or following years)
Emrah Ero─člu -emrah.eroglu@sabanciuniv.edu,
English
Undergraduate
--
Formal lecture,Interactive lecture,Laboratory
Interactive,Communicative,Discussion based learning,Task based learning,Case Study
Click here to view.

CONTENT

A general introduction to basic techniques used in characterization and separation of biological systems and molecules. Topics include microscopy, centrifugation, liquid chromatography methods, spectroscopy and electrophoresis. Lab sessions will give a chance to use these techniques individually.

OBJECTIVE

To teach students the basic concepts of bioanalytical techniques in both, theory and practice, which are relevant to biologists and bioengineers but also students from different fields.

LEARNING OUTCOME

Upon completion of the course, students will have a general knowledge of basic laboratory techniques for characterization and separation of biological systems and molecules. Students would be able to perform basic laboratory techniques.


Module 1 Microscopy: Students will have a deeper understanding of both, theory and application in the following topics:

Basic Concepts in Microscopy
Light microscopy
Fluorescence microscopy
Confocal Microscopy
Live-cell imaging and Sensor techniques

Module 2 Electrophoresis Techniques: Students will learn how to use Gel electrophoresis techniques for analysis of nucleic acids and proteins for both, diagnostic purposes and preparative purposes, which includes:

Basic Concepts in Electrophoresis
Horizontal and Vertical Electrophoresis
2D Gel Electrophoresis and Protein Detection Methods
Electrophoresis of Nucleic Acids

Module 3 Centrifugation Techniques:

Basic Concepts of Centrifugation Techniques
Types of Centrifuges and analytical ultracentrifugation techniques
Separation methods and preparative ultracentrifuges
Types of rotors

Module 4 Spectroscopic Techniques:

Introduction and basic concepts of Spectroscopic Techniques
UV-VIS Spectroscopy
Infrared and fluorescence spectroscopy
Circular dichroism (CD) spectroscopy
NMR Spectroscopy and X-ray crystallography
Atomic Spectroscopy and Mass spectroscopy

Module 5 PCR Techniques:

Principles of Polymerase Chain reaction
Preparative and Diagnostic PCR
DNA Sequencing methods
Sanger Sequencing, Next Generation Sequencing Methods

Module 6 Chromatography and Mass spectrometry:

Basic Principles of Chromatography
Thin layer chromatography
Liquid Chromatography
Gas Chromatography
Principles of MALDI-TOF
Electrospray Ionization Mass Spectrometry

PROGRAMME OUTCOMES


1. Understand the world, their country, their society, as well as themselves and have awareness of ethical problems, social rights, values and responsibility to the self and to others. 2

2. Understand different disciplines from natural and social sciences to mathematics and art, and develop interdisciplinary approaches in thinking and practice. 5

3. Think critically, follow innovations and developments in science and technology, demonstrate personal and organizational entrepreneurship and engage in life-long learning in various subjects. 3

4. Communicate effectively in Turkish and English by oral, written, graphical and technological means. 3

5. Take individual and team responsibility, function effectively and respectively as an individual and a member or a leader of a team; and have the skills to work effectively in multi-disciplinary teams. 3


1. Possess sufficient knowledge of mathematics, science and program-specific engineering topics; use theoretical and applied knowledge of these areas in complex engineering problems. 3

2. Identify, define, formulate and solve complex engineering problems; choose and apply suitable analysis and modeling methods for this purpose. 2

3. Develop, choose and use modern techniques and tools that are needed for analysis and solution of complex problems faced in engineering applications; possess knowledge of standards used in engineering applications; use information technologies effectively. 3

4. Ability to design a complex system, process, instrument or a product under realistic constraints and conditions, with the goal of fulfilling specified needs; apply modern design techniques for this purpose. 1

5. Design and conduct experiments, collect data, analyze and interpret the results to investigate complex engineering problems or program-specific research areas. 5

6. Knowledge of business practices such as project management, risk management and change management; awareness on innovation; knowledge of sustainable development. 1

7. Knowledge of impact of engineering solutions in a global, economic, environmental, health and societal context; knowledge of contemporary issues; awareness on legal outcomes of engineering solutions; understanding of professional and ethical responsibility. 2


1. Comprehend key concepts in biology and physiology, with emphasis on molecular genetics, biochemistry and molecular and cell biology as well as advanced mathematics and statistics. 4

2. Develop conceptual background for interfacing of biology with engineering for a professional awareness of contemporary biological research questions and the experimental and theoretical methods used to address them. 4


1. Applying fundamental and advanced knowledge of natural sciences as well as engineering principles to develop and design new materials and establish the relation between internal structure and physical properties using experimental, computational and theoretical tools. 2

2. Merging the existing knowledge on physical properties, design limits and fabrication methods in materials selection for a particular application or to resolve material performance related problems. 1

3. Predicting and understanding the behavior of a material under use in a specific environment knowing the internal structure or vice versa. 1


1. Use mathematics (including derivative and integral calculations, probability and statistics, differential equations, linear algebra, complex variables and discrete mathematics), basic sciences, computer and programming, and electronics engineering knowledge to (a) Design and analyze complex electronic circuits, instruments, software and electronics systems with hardware/software. or (b) Design and analyze communication networks and systems, signal processing algorithms or software 2

ASSESSMENT METHODS and CRITERIA

  Percentage (%)
Final 30
Midterm 30
Group Project 40

RECOMENDED or REQUIRED READINGS

Textbook

Bioanalytics: Analytical Methods and Concepts in Biochemistry and Molecular Biology
Friedrich Lottspeich (Editor), Joachim W. Engels (Editor)
ISBN: 978-3-527-33919-8

Readings

Course slides updated annually.